Y-larvae, mysterious crustaceans associated to barnacles, could also be parasitic and are key to understanding barnacle evolution.
When most individuals consider barnacles, they think about shell-like organisms clinging to boats, docks, and even whales. But some barnacles go far past passive attachment — they’ll really invade and take over their hosts.
“As a substitute of gluing themselves to a rock or one thing, they glue themselves to a number, typically a crab, and so they inject themselves into that host, and dwell their complete life as a root community rising by means of their host. It’s nearly like a fungal community or plant root system. They haven’t any actual physique in the way in which that we consider animal our bodies,” explains James Bernot, an assistant professor within the Division of Ecology and Evolutionary Biology at UConn.
Bernot and a global group of collaborators — together with lead creator Niklas Dreyer from the Pure Historical past Museum of Denmark and Biodiversity Analysis Middle Academia Sinica in Taiwan, Jørgen Olesen from the Pure Historical past Museum of Denmark, Gregory Kolbasov from Moscow College, Jens Høeg from the College of Copenhagen, and Ryuji Machida and Benny Chan from the Biodiversity Analysis Middle Academia Sinica — just lately printed a examine in Current Biology on a puzzling group of crustaceans that may help resolve one of marine biology’s enduring mysteries.
The mystery of y-larvae
Barnacles are crustaceans, like crabs and shrimp, and have evolved unusual survival strategies. After a free-swimming larval phase, they spend the rest of their lives permanently attached to a chosen surface.
One especially mysterious group, known as “y-larvae” or Facetotecta, looks like juvenile barnacles. They have been documented in plankton samples since the 1800s, but no one has ever identified their adult stage. Bernot notes that this unresolved question remains central, though the team’s new research brings science closer to an answer.
To investigate where y-larvae belong on the crustacean family tree, the researchers gathered more than 3,000 specimens and examined their genes by sequencing the transcriptome — the set of expressed RNA molecules that reflects which genes are active.
Genetic analysis and hidden lives
“We were finally able to confirm, in the realm of big data science, that they are, in fact, related to barnacles, but they aren’t closely related to any of the other parasitic barnacles. This was interesting to test by building a giant tree of life for all the crustaceans, then adding this little branch of y-larvae, this very unknown group, to that bigger tree, and we saw that they are related to barnacles, but more as distant cousins,” says Bernot.
Though not closely related to parasitic barnacles, these crustaceans are also likely parasitic because they have some structures in common with their parasitic cousins, says Bernot, including antennae with claws that may be used to hook onto their host.
“One of the best pieces of evidence we have that y-larvae become parasitic is that if we expose them to crustacean growth hormone, they will hatch out of their little swimming larval shape into a small slug-like body, which is similar to what parasitic barnacles do when they enter a host,” says Bernot. “The fact that if we give them hormones, they also molt into a slug-like thing, suggests they go on to be parasitic somewhere, but we still don’t know what host they end up in. Being hidden inside another animal’s body could explain why we haven’t found the adult stage of y-larvae yet.”
Although these crustaceans are unusual and largely unknown with only 17 species described so far, Bernot says some of his co-authors found more than 100 new and different species from a single harbor in Japan. There is more to learn about these enigmatic animals.
Evolutionary strategies and ecosystem roles
“We were finally able to confirm, in the realm of big data science, that they are, in fact, related to barnacles, but they aren’t closely related to any of the other parasitic barnacles. This was interesting to test by building a giant tree of life for all the crustaceans, then adding this little branch of y-larvae, this very unknown group, to that bigger tree, and we saw that they are related to barnacles, but more as distant cousins,” says Bernot.
Although they are only distantly related to parasitic barnacles, the evidence suggests y-larvae are also parasitic. They share certain traits with parasitic barnacles, including clawed antennae that may help them latch onto a host.
“One of the best pieces of evidence we have that y-larvae become parasitic is that if we expose them to crustacean growth hormone, they will hatch out of their little swimming larval shape into a small slug-like body, which is similar to what parasitic barnacles do when they enter a host,” says Bernot. “The fact that if we give them hormones, they also molt into a slug-like thing, suggests they go on to be parasitic somewhere, but we still don’t know what host they end up in. Being hidden inside another animal’s body could explain why we haven’t found the adult stage of y-larvae yet.”
Despite being poorly understood, with only 17 described species, y-larvae may be far more diverse than previously thought. Bernot points out that some of his colleagues identified more than 100 distinct species from just a single harbor in Japan, suggesting much remains to be discovered about these unusual animals.
Ingenious barnacle adaptations
Different species of barnacles use different strategies when they become sessile adults. Besides living on inanimate objects, those that live on animals like whales are not considered parasitic because they are essentially hitching a ride and do not feed on their host. Others attach to the host and have structures that they use to feed on the host. Understanding the evolution of these different strategies is important, and Bernot says that a project they are currently working on involves building the evolutionary tree of all barnacles to observe and understand some of the evolutionary patterns.
“A big question is, what is it about barnacles that has given them so much variability over evolutionary time to take on so many different shapes and forms and lifestyles? They have come up with incredibly ingenious strategies for making their ways of life, and often their ways of life seem very bizarre to us, but they have clearly been very successful,” says Bernot. “These animals have been around for hundreds of millions of years and there are several thousand species of them, so they have come up with some really amazing solutions to complex problems.”
Some of those solutions could also help humans. For example, Bernot says, there is a lot of interest in trying to better understand barnacle glues.
“They glue themselves to docks, they glue themselves to boats, and that is a problem. The Navy spends millions of dollars on additional fuel because barnacles on their ships cause additional drag. Also having more powerful glues that can dry underwater would be very useful for mechanical reasons, but maybe also for dentistry and things like that,” says Bernot. “There could be a lot of applications if we can better understand some of these amazing solutions that barnacles have evolved.”
Reference: “Phylogenomics of enigmatic crustacean y-larvae reveals multiple origins of parasitism in barnacles” by Niklas Dreyer, James P. Bernot, Jørgen Olesen, Gregory A. Kolbasov, Jens Thorvald Høeg, Ryuji J. Machida and Benny K.K. Chan, 21 July 2025, Current Biology.
DOI: 10.1016/j.cub.2025.06.007
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